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To address these challenges, emerging strategies such as nanoencapsulation and advanced delivery systems have been developed to enhance bioavailability and improve targeting mechanisms [28, 94, 216]

Commonly used target detection methods such as immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) are subject to inter-laboratory variability, ambiguous cutoff definitions, and limited quantitative precision [304, 305]

Prevention is often the best strategy

10.1038/s41401-020-00525-z 33 ChoiH.BaeS

Ideal candidates include individuals experiencing unexplained fatigue unresponsive to sleep and stress management, vegetarians and vegans lacking dietary B12 sources, adults over 50 with age-related absorption decline, patients with digestive disorders affecting nutrient absorption, those taking medications that deplete B12, and anyone seeking natural energy support without stimulants

Furthermore, it is evident that EVs can induce oxidative stress by delivering reactive oxygen species or by modulating the antioxidant defenses of recipient cells