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In brief, bones were fully decalcified in 14% EDTA at a pH of 7.4 for 2 weeks before embedding in optimal cutting temperature mounting media (Fisher HealthCare, 23-730-571) and sectioning at 50 m on a cryostat (Leica)

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Challenges and Considerations While genetic testing offers numerous benefits, it is not without challenges

Flavonoids reduce the effects of harmful bacteria

It is currently in phase IIb trial aiming at recruiting 185 patients (NCT02665364)

For example, monoclonal antibodies to be used may be made by the hybridoma method first described by Koehler et al., Nature, 256: 495 (1975), or may be made by recombinant DNA methods (see, e.g., U.S

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