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In the study, the team exposed mice to cigarette smoke, and then cohoused them with air-exposed mice (control) and germ-free mice

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The difference between estimates calculated from responses of the two test groups were then used to produce an adjustment factor that was applied to 2018 and 2019 responses, based on original design levels

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There are various methods that aim to speed up the steeping processbut these are a subject of much controversy in the DIY e-juice community