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We showed that the PfGrx-dependent oxidation and reduction of roGFP2 both efficiently occur via a monothiol mechanism (Fig

Abbreviations AR: Antibiotic resistances CAMHB: Cation-adjusted Mueller-Hinton cation-adjusted Mueller-Hinton broth C : d Donor cell drug concentration CDC: Center for Disease Control and Prevention CLSI: Clinical and Laboratory Standards Institute C : n Characteristic ratio D : i Diffusion coefficient GSH: Glutathione GSH-PEG: Glutathione-conjugated polyethylene glycol GST: Glutathione-S-transferase I : Ionic strength IMF: Intermolecular force j : ss Flux of drug through membrane at steady state K : i Partition coefficient l : B ond length along the polymer backbone L : Membrane thickness LMS: Low molecular weight salt m : 0 Mass of the dried hydrogel M : c Molecular weight between crosslinks m : d,a Mass of dry hydrogel in air m : i Initial relaxed hydrogel mass MIC: Minimum inhibitory concertation M : n Number average molecular weight M : r Molecular weight of the repeating unit m : r,b Mass of relaxed hydrogel in butanol MRSA: methicillin-resistant Staphylococcus aureus m : s,b Mass of swollen hydrogel in butanol m : t Swollen mass at a given time p : Statistical p value PBS: Phosphate buffered saline PEG: Poly(ethylene glycol) PEGDA: Poly(ethylene glycol) diacrylate P : i Permeability coefficient q : Hydrogel mass swelling ratio SSTI: Skin and soft tissue infection t : lag Lag time to steady state conditions UV: Ultraviolet v : S pecific volume of the polymer V 1 : Molar volume of the swelling agent, water m: Change in mass (%) : 2,r Polymer volume fraction in the relaxed state : 2,s Polymer volume fraction in the swollen state : Mesh size of the hydrogel : b Density of 1-butanol : p Density of polymer : Flory polymer-solvent interaction factor References Tun K, Shurko JF, Ryan L, Lee GC

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Yes this is one of the strongest established applications of metabolomics in drug discovery

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