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A 1 mL aliquot of each urine sample was first incubated with 200 uL ethyl hydroxylamine hydrochloride (75g/L) at 60oC for 30 minutes to convert keto-acids to their ethoxime derivatives

For the targeted sulfur metabolite quantification approach, the previously established LC-MS conditions were applied 99 with selected reaction monitoring (MRM) using an Ultimate 3000 UPLC system coupled to a Thermo Finnigan TSQ Quantum equipped with HESI (Thermo Fisher Scientific, Waltham, MA)

Individual experiences vary based on hydration, nutrition, sun exposure and general skincare habits

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