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Western Blotting Analysis Proteins from the whole kidney lysates were separated on a 10% SDS-polyacrylamide gel, transferred onto PVDF membrane, blocked in 5% BSA for 60min, and then primary antibodies against KIM-1, IL-1, TNF-, Ferritin, FPN, xCT, and p53 at the dilution of 1:1,000, as well as 4-HNE, GPX4, and -actin at the dilution of 1:4,000 were probed at 4C overnight

Cavernous artery flow and end-diastolic velocity were normal: peak systolic velocity was 74 cm/seconds (bilaterally)

El-Kholy, and M

10.12182/20241160603 8 ChikW.-I.ZhuL.FanL.-L.YiT.ZhuG.-Y.GouX.-J.et al (2015)

Once you have that data, you can introduce a second compound and accurately attribute any new effects to the synergy of the stack