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The supernatants absorbance was recorded at 665, 647, and 461 nm using a UV-Visible spectrophotometer (SpectramaxPlus 384, Molecular Devices, USA)

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22 However, joint aspiration is rarely necessary in practice unless there is a high suspicion of another cause, e.g

Possible risk factors for hypophosphatemia include a history of gastrointestinal disorders associated with malabsorption of fat-soluble vitamins or phosphate, inflammatory bowel disease, concurrent or prior use of medications that affect proximal renal tubular function, hyperparathyroidism, vitamin D deficiency, malnutrition, and hereditary hemorrhagic telangiectasia (HHT or Osler-Weber-Rendu syndrome)